Evaluation and comparative analysis on reduction of Globalfiler™ reaction volume in low template samples

In forensic genetic laboratories, low template DNA (LTDNA) samples can be a challenge in the attempting to obtain genetic profiles with the most information possible. In this study, volume reduction on amplification reaction was tested in the kit Globalfiler (TM), with the intend to optimize the results in LTDNA samples. To carry out this study, several samples extracted with PrepFiler (R) were diluted successively to very low concentration values. All samples and respective dilutions were amplified with Globalfiler (TM), for both reaction volumes of 12,5 mu L and 25 mu L. Capillary electrophoresis was performed on the ABI PRISM (R) 3500 Genetic Analyzer and the HID files generated were analysed using GeneMapper (R) ID-X v. 1.4. Forensic DNA samples with very low values of DNA concentration were also tested in the same conditions. The results indicate that the reduction of volume in amplification reaction with Globalfiler T can often produce higher quality profiles, resulting as an alternative to enhance allelic recovery.