油菜素内酯对桔梗组培幼苗生长和生理特性的影响 The Effects of Brassinosteroid on Growth and Physiological Properties of Platycodon grandiflorum Seedlings in Tissue Culture

本文研究了生根培养基中添加不同浓度的油菜素内酯(BR)对桔梗组培幼苗生长和生理特性的影响，外源BR的浓度为0, 0.025, 0.05, 0.10, 0.20, 0.40 mg/L。结果表明：幼苗不定根的条数、幼苗节数、叶绿体色素含量、POD和SOD活性均随BR浓度的增加呈先增高后降低趋势，在0.10 mg/L处理达到最大值，说明该处理有利于培养健壮幼苗，提高叶绿素含量和增强幼苗的抗性。 In this study, the effects of Brassinosteriod (BR) on growth and physiological properties of Platycodon grandiflorum seedlings were conducted by adding exogenous BR in the root inducing medium, and the concentration of exogenous BR is 0, 0.025, 0.05, 0.10, 0.20, 0.40 mg/L. The results showed that the number of adventitious root and node, chlorophyll content, activity of POD and SOD in P. grandiflorum seedlings were increased first and then decreased, and the maximum value in 0.1 mg/L treatment, respectively. Obviously, BR application could significantly increase the chlorophyll content, the resistance of the P. grandiflorum leaves, and cultivate the health and strong seedlings in the 0.1 mg/L treatment. The results provided theoretical foundation in seedling culture of P. grandiflorum.