PK-15 Hücrelerinde Domuz Transmissible Gastroenteritis Virüsüne Karşı Rekombinant Domuz İnterferon-α’nın Antiviral Aktivitesi

A recombinant porcine interferon alpha (rPoIFN-alpha) has been developed and patented previously (Chinese patent number ZL200810020180.4). In the current study, we investigated the inhibitory effects of the rPoIFN-alpha on the propagation of porcine transmissible gastroenteritis virus (TGEV) at different doses in porcine kidney cell line (PK-15). To quantitatively determine the inhibition of viral growth by rPoIFN-alpha, TCID50 assay, plaque formation assay, real-time qRT-PCR, western blot and immunofluorescence assay were adopted to evaluate the changes of viral infectious particles, viral genome copy numbers and viral protein expression levels respectively. The results demonstrated that all the three batches of the rPoIFN-alpha tested inhibited TGEV-induced cytopathic effect in PK-15 cells with very similar potency. rPoIFN-alpha inhibited TGEV proliferation more strongly than human IFN-alpha product. The inhibitory activity of rPoIFN-alpha on TGEV growth in culture was dose dependent, and the activity was gradually reduced with the decreasing of the concentration of rPoIFN-alpha.