The role of α-tocopherol transfer protein-tocopherol transfer protein (α-TTP) for the uptake of vitamin E in the prevention of glutamate induced injury in neuronal cells

Event Abstract Back to Event The role of α-tocopherol transfer protein-tocopherol transfer protein (α-TTP) for the uptake of vitamin E in the prevention of glutamate induced injury in neuronal cells Huzwah Khaza'Ai1*, Thilaga R. Selvaraju2, Mohd Sokhini Abd Mutalib3 and Sharmili Vidyadaran4 1 Universiti Putra Malaysia, Department of Biomedical Science, Faculty of Medicine and Health Sciences,, Malaysia 2 Melaka Manipal Medical College , Foundation of Science, Malaysia 3 Universiti Putra Malaysia, Deparment of Nutrition and Dietetic, Faculty of Medicine and Health Sciences, Malaysia 4 Universiti Putra Malaysia, Department of Pathology, Faculty of Medicine and Health Sciences, , Malaysia Vitamin E composed of eight different isomers of a, ß, γ, δ -tocopherols and tocotrienols. Human α-TTP is a cytosolic liver protein that plays a central role in vitamin E homeostasis with strong affinity to α-tocopherol. Despite of discriminated by α-TTP, α-tocotrienol provides the most potent neuroprotection amongst vitamin E. Tocotrienols has been shown to possess powerful antioxidant properties that can reduce effects of oxidative stress at nanomolar concentration. In view of the fact that the source of vitamin E for Malaysian is primarily from tocotrienol, therefore understanding of its uptake is rather crucial to ensure its benefit to the consumer. The aim of this study is to elucidate the behaviour of α-TTP in the condition with the absence of tocopherol, whether α-TTP will seek for tocotrienol. We assume that α-TTP is capable to take up tocotrienol in the condition with less availability of tocopherol. Cells were then treated with different ratio of Tocopherol (T) : Tocotrienol Rich fraction (TRF) (%): (100 T:0 TRF), (75 T:25 TRF), (50 T:50 TRF), (25T:75TRF), and (0T:100 TRF) and incubated for 12 and 24 hours respectively. RNA extraction was performed followed by reverse transcribe to cDNA. The expression of α-TTP upon treatment with different ratio of T:TRF was determined by Real Time Polymerase Chain Reaction (PCR) which amplify the DNA segment using α-TTP primer and GAPDH primer as a housekeeping gene and SYBR Green as a probe. The result was analyzed with statistical analysis one way ANOVA and post hoc Tukey test and p = 0.05 is considered statistical significant. α-TTP is capable to take up tocotrienol in the condition with less availability of tocopherol and we can postulate that α-TTP is not only responsible for tocopherol uptake but it is also involved in the uptake of other vitamin E isomers. Keywords: Gene Expression, Vitamin E, Neuron, injury, α-Tocopherol transfer protein Conference: 14th Meeting of the Asian-Pacific Society for Neurochemistry, Kuala Lumpur, Malaysia, 27 Aug - 30 Aug, 2016. Presentation Type: Poster Presentation Session Topic: 14th Meeting of the Asian-Pacific Society for Neurochemistry Citation: Khaza'Ai H, Selvaraju TR, Abd Mutalib M and Vidyadaran S (2016). The role of α-tocopherol transfer protein-tocopherol transfer protein (α-TTP) for the uptake of vitamin E in the prevention of glutamate induced injury in neuronal cells. Conference Abstract: 14th Meeting of the Asian-Pacific Society for Neurochemistry. doi: 10.3389/conf.fncel.2016.36.00122 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 04 Aug 2016; Published Online: 11 Aug 2016. * Correspondence: Dr. Huzwah Khaza'Ai, Universiti Putra Malaysia, Department of Biomedical Science, Faculty of Medicine and Health Sciences,, Serdang, Selangor, Malaysia, huzwah@upm.edu.my Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Huzwah Khaza'Ai Thilaga R Selvaraju Mohd Sokhini Abd Mutalib Sharmili Vidyadaran Google Huzwah Khaza'Ai Thilaga R Selvaraju Mohd Sokhini Abd Mutalib Sharmili Vidyadaran Google Scholar Huzwah Khaza'Ai Thilaga R Selvaraju Mohd Sokhini Abd Mutalib Sharmili Vidyadaran PubMed Huzwah Khaza'Ai Thilaga R Selvaraju Mohd Sokhini Abd Mutalib Sharmili Vidyadaran Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.