Presence of BlaCTX-M and BlaTEM among environmental Enterobacteriaceae isolated from the São Domingos River, Peniche, Portugal

Event Abstract Back to Event Presence of BlaCTX-M and BlaTEM among environmental Enterobacteriaceae isolated from the São Domingos River, Peniche, Portugal Cátia Costa1, Marco Simões1, Joana Ribeiro1, Joana Fernandes1, Cláudia Lopes1, Americo Rodrigues1 and Maria J. Campos1* 1 MARE – Marine and Environmental Sciences Centre, ESTM, Instituto Politécnico de Leiria, Portugal Nowadays humanity faces an emerging threat to general public health since antibiotic resistant bacteria are increasing due to the acquisition of molecular resistance determinants. This widespread in bacterial resistance is most likely related to the abusive and unnecessary use of antibiotics such as β-Lactams. Without a doubt, this practice promotes the selection of resistant strains; including those capable of producing different extended spectrum β-lactamases (ESBLs). Typically, susceptible bacteria do not possess the necessary genetic machinery to survive in the presence of β-Lactams, relying on the horizontal transfer of resistance plasmids. Environmental contamination with bacteria originated from urban wastewaters and effluents from livestock farms can be a source of resistance plasmids capable of being acquired by susceptible bacteria. Thus, it becomes essential to conduct environmental studies regarding the occurrence of resistant strains present in natural watercourses. The objective of this study was to determine the presence of bacterial molecular determinants encoding the ESBL BlaCTX-M and blaTEM in the S. Domingos River, Peniche, Portugal. A selective media to Enterobacteriaceae with the antimicrobial cefotaxime was used for bacterial isolation and the presence of blaCTX-M and blaTEM was evaluated by PCR. It was concluded that both genes were present in several bacteria, suggesting that the resistance to β-lactams is spreading through environment areas. This fact poses a noteworthy threat to the public health, as it shows that drug resistant bacteria are present in watercourses, with the potential to transmit resistance genes to native bacteria. Acknowledgements This study had the support of Fundação para a Ciência e Tecnologia (FCT), through the strategic project UID/MAR/04292/2013 granted to MARE. Keywords: Enterobacteriaceae, ESBL, CTX-M, TEM, Betalactamases, antimicrobial resistance Conference: IMMR | International Meeting on Marine Research 2016, Peniche, Portugal, 14 Jul - 15 Jul, 2016. Presentation Type: Poster presentation Topic: Biodiversity, Conservation and Coastal Management Citation: Costa C, Simões M, Ribeiro J, Fernandes J, Lopes C, Rodrigues A and Campos MJ (2016). Presence of BlaCTX-M and BlaTEM among environmental Enterobacteriaceae isolated from the São Domingos River, Peniche, Portugal. Front. Mar. Sci. Conference Abstract: IMMR | International Meeting on Marine Research 2016. doi: 10.3389/conf.FMARS.2016.04.00073 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 07 Jun 2016; Published Online: 13 Jul 2016. * Correspondence: Dr. Maria J Campos, MARE – Marine and Environmental Sciences Centre, ESTM, Instituto Politécnico de Leiria, Peniche, Portugal, mcampos@ipleiria.pt Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Cátia Costa Marco Simões Joana Ribeiro Joana Fernandes Cláudia Lopes Americo Rodrigues Maria J Campos Google Cátia Costa Marco Simões Joana Ribeiro Joana Fernandes Cláudia Lopes Americo Rodrigues Maria J Campos Google Scholar Cátia Costa Marco Simões Joana Ribeiro Joana Fernandes Cláudia Lopes Americo Rodrigues Maria J Campos PubMed Cátia Costa Marco Simões Joana Ribeiro Joana Fernandes Cláudia Lopes Americo Rodrigues Maria J Campos Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.