Estudio del patrón de expresión de microRNAs en el hueso osteoporótico

espanolObjetivos: Identificar microRNAs (miRNAs) diferencialmente expresados en muestras oseas con fractura osteoporotica respecto a huesos sanos. Material y metodos: Se extrajo RNA total a partir de hueso trabecular fresco del cuello femoral de mujeres sometidas a reemplazo de cadera, ya sea debido a fractura osteoporotica (n=6) o por artrosis en ausencia de osteoporosis (segun la DMO) (n=6). Las muestras se hibridaron en un array de miRNAs y se realizaron diagramas de PCA y de mapa de calor. Para la comparacion de los niveles de expresion, se fijo como significativo un umbral de cambio de u003e1,5 veces y un valor p EnglishObjectives: To identify microRNAs (miRNAs) differentially expressed in bone samples with osteoporotic fracture compared with healthy bones. Methods: Total RNA was extracted from fresh trabecular bone of the femoral neck of women undergoing hip replacement surgery, either because to osteoporotic fracture (n=6) or in the absence of osteoarthritis osteoporosis (based on BMD) (n=6). The samples were hybridized on an array of miRNAs and PCA diagrams and heat map were made. To compare expression levels, u003e1.5 times and a value p These two miRNAs were detected in cultures of primary osteoblasts, although they did not maintain the same pattern of expression in total bone samples. Conclusions: We have shown that there are differences in the expression of miRNAs in samples with osteoporotic fracture. This opens prospects for research and design of new therapies.