Calcium channel α_1 expression and significance of axillary sweat glands in primary palmar hyperhidrosis along with axillary hyperhidrosis patients

Objective To investigate the expression level of the calcium channelα1 in patients with primary palmar hyperhidrosis along with axillary hyperhidrosis.Methods We choosed 28 patients who were diagnosed as primary palmar hyperhidrosis along with axillary hyperhidrosis from October 2011 to September 2012 in our hospital and 8 cases of normal control group during this period.When the patients were operated,we cut out a block of axillary skin sweat gland tissue.We observed sweat gland structure by light and electron microscope,and used Real time PCR technology and Western blot protein imprinting technology to determine the expression level of calcium channelα1 in patients with primary palmar hyperhidrosis along with axillary hyperhidrosis.Results Sweat gland structure had no difference by light microscope.The sweat gland secretion granules of experimental group were obviously higher than that of control group by 12 500 times electron microscopy.The expression levels of calcium channelα1 mRNA and its protein were higher in patients with primary palmar hyperhidrosis along with a axillary hyperhidrosis than those in normal group,and the difference was statistically significant(P0.05).Conclusion Sweat gland secretion granules obviously increased,the expression level of calcium channelα1 increased in the axillary sweat gland tissues of the patients with primary palmar hyperhidrosis along with axillary hyperhidrosis,these might be one of the causes of primary palmar hyperhidrosis.