Formulation, characterization and anti-tumor synergistic evaluation of Nano emulsion formulation containing Curcumin and Brucea javanica oil

Objective: The aim of the study was to prepare and in more details improve antitumor effect by nanoemulsion (NE) loaded with curcumin (CUR) and brucea javanica oil (BJO) together. Materials and Methods: Solubility of CUR and BJO in various vehicles were conducted in a shaking incubator. Nanoemulsion was prepared by pseudo-ternary phase diagrams to obtain the concentration range of components. The physicochemical and biological characteristics of the NE was studied by size and zeta potential, morphologies, physical stability, in vivo antitumor activity and vivo pharmacokinetics. Key findings Results: The combination of BJO and CUR in optimized NE formulation exhibited excellent stability. Besides, it showed a synergy antitumor effect and enhanced bioavailability. Conclusions: The combination of BJO and CUR in optimized NE was successfully formulated. BJO used as the oil phase produced an antitumor synergistic effect with CUR was investigated by anti-tumor activity experiment. The dissolution rate and oral bioavailability were also improved compared with pure CUR