ARTSY-J: Convenient and precise measurement of (3)JHNHα couplings in medium-size proteins from TROSY-HSQC spectra.

Julien Roche,Jinfa Ying,Yang Shen,Dennis A. Torchia,Ad Bax

Journal of Magnetic Resonance（2016）

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Abstract

•A convenient and accurate method for measurement of 3JHNHα is described.•The 2D method is based on the TROSY principle and yields excellent resolution.•The effect of 1H–1H cross relaxation can be accounted for by linear scaling.•The method also yields phi angle information for Gly residues.

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Key words

Backbone torsion angle,Gly,Karplus equation,TROSY,GB3,HIV-1 Protease,Protein NMR

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