Regio-selective Reduction of Oxysophoridine by Microorganism Isolated from Soil

Oxysophoridine (OSR), an alkaloid extracted from a traditional Chinese medicine, attracted more and more attention because of remarkable biological activities. Biocatalysis of OSR by 86 stains including soil microbes and endophytic fungi were investigated. Incubation of OSR with soil bacteria T003 identified as Escherichia coli could lead to only one reductive product with highly regio-selectivity. The structural elucidations of transformation product were achieved mainly by the NMR spectroscopic methods and references. Effect of pH, substrate concentration, conversion time and strain concentration was studied, the optimization of reaction was analyzed by LC-MS. The production of sophoridine (SR) from OSR was highest at pH 7.0, 36 h of incubation and in presence of 625 mu g/mL substrate concentration, 50 mu L/mL strain concentration respectively. Biocatalysis was considered the alternatives to preparation of sophoridine with green and sustainable synthetic processes except for less time-consuming and more environmentally friendly.