Time‐dependent Expression of MMP‐2 and TIMP‐2 after Rats Skeletal Muscle Contusion and Their Application to Determine Wound Age
JOURNAL OF FORENSIC SCIENCES(2016)
摘要
The ability to determine vitality and estimate the survival period after a wound is critical in routine forensic practice. The mRNA levels of MMP-2 and TIMP-2 were examined using quantitative real-time RT-PCR to determine the age of a wound. Furthermore, the colocalization of them with Macrophage Marker, respectively, was detected by double immunofluorescence, and a standardized rat model of skeletal muscle contusion was established. In the antemortem contused groups, a large number of macrophages showed positive staining for MMP-2 and TIMP-2, and the expression of MMP-2 and TIMP-2 mRNA increased sharply at 3 days postinjury, with relative quantities of 5.75 and 2.98. No samples in the other groups showed relative quantities of >5.75 and 2.98; therefore, relative quantities exceeding 5.75 and 2.98 were strongly indicated 3 days after contusion. In addition, there was a significant decrease in the relative quantity in the postmortem contused groups, indicating that they were useful for diagnosing vitality.
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关键词
forensic science,forensic pathology,wound age determination,matrix metalloproteinase-2,tissue inhibitor of metalloproteinase-2,macrophage,quantitative real-time RT-PCR
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