A fatty acid profiling method using liquid chromatography–high resolution mass spectrometry for improvement of assisted reproductive technology

•High-resolution mass spectrometry was applied for in vitro fertilization.•Fatty acid uptake by preimplanted embryo from culture medium was analyzed.•Samples are culture media after use, and therefore this method is non-invasive.•Vigorous embryonic uptake of docosahexaenoic acid indicates proper growth.•High-resolution mass spectrometry is promising in in vitro fertilization.