Research on Response Surface Optimization of Culture Medium for Antibacterial Substances Produced by Bacillus Amyloliquefaciens GN59

Single factor optimization experiment and response surface optimization experiment were designed to research the influence of different culture medium components to bacteriostatic active substances produced by bacillus amyloliquefaciens GN59 strain, and a culture medium formula suitable for the GN59 strain to produce the bacteriostatic active substances was screened. In the single factor experiment, the optimal formula was: glucose 10.0g/L, K2HPO4·3H2O 1.2 g/L, KH2PO4 1.0 g/L, (NH4)2SO4 2.00 g/L, Na3C6H5O7·3H2O 0.9 g/L, and MgSO4·7H2O 0.15 g/L, and the bacteriostatic activity was improved by 14.32% in comparison with that before optimization. In the response surface optimization experiment, the optimal culture medium components were glucose 6.15 g/L, K2HPO4·3H2O 1.2 g/L, KH2PO4 1.0 g/L, (NH4)2SO4 2.50 g/L, Na3C6H5O7·3H2O 1.01 g/L, and MgSO4·7H2O 0.15 g/L, the bacteriostatic activity was improved by 31.18% in comparison with that before optimization, and meanwhile, the product cost was lowered, and batch low-cost and efficient fermentation production of the bacillus amyloliquefaciens GN59 strain was realized.