Antibacterial effects of Lactobacillus isolates of curd and human milk origin against food-borne and human pathogens

This study was undertaken to assess the antibacterial efficacy of lactobacilli isolated from curd and human milk samples. Identities of thirty-one different lactobacilli (20 from curd and 11 from human milk) were confirmed by genus-specific PCR and 16S rRNA-based sequencing. These strains belonged to five species, Lactobacillus casei , L. delbrueckii , L. fermentum , L. plantarum, and L. pentosus . Antibacterial activities of cell-free supernatants (CFSs) of all the Lactobacillus isolates were estimated through standard agar-well diffusion assay, against commonly occurring food-borne and clinically important human pathogens. None of the lactobacilli cell-free supernatant (CFS) exhibited inhibitory activity against four pathogens, namely Staphylococcus aureus , Listeria monocytogenes, Escherichia coli, and Klebsiella pneumoniae. Bacillus cereus , Salmonella enterica serovar Typhi, and Shigella flexneri were moderately inhibited by majority of CFSs, whereas, weak activity was observed against Pseudomonas aeruginosa and Proteus mirabilis . CFS of some of the curd isolates displayed antagonistic activity against Streptococcus mutans ; however, human milk lactobacilli did not displayed any inhibitory activity against them. As expected, Nisin (Nisaplin ® ) showed inhibitory activity against Gram-positive, S. aureus , B. cereus, and L. monocytogenes. Interestingly, few of the examined CFSs exhibited inhibitory activities against both Gram-positive and Gram-negative pathogens. Findings from this study support the possibility to explore the tested lactobacilli and their CFSs as natural bio-preservatives, alone or in combination with approved bacteriocins in food and pharma formulations after validating their safety.