PM 2.5 affects establishment of immune tolerance in newborn mice by reducing PD-L1 expression

This study was conducted to determine whether exposure to particulate matter 2.5 (PM 2.5 ) affects the immune tolerance of neonatal mice via the regulation of PD-L1 expression. One-week-old BALB/c mice were exposed to PM 2.5 for 8 days. From day 8 to day 18, the mice were treated with 5 μg house dust mite (HDM) (i. n.) every two days. Adenovirus-carried PD-L1 overexpression vectors were infected into mice via nasal inhalation 6 days after exposure to PM 2.5 . Airway hyper-responsiveness (AHR) was examined in mice 19 days after exposure to PM 2.5 , and the related parameters of airway inflammation were studied on day 22. Co-exposure to PM 2.5 and HDM reduced PD-L1 expression but greatly increased infiltration of inflammatory cells, which was reversed by PD-L1 overexpression. Co-exposure to PM 2.5 and HDM also elevated serum IL-4, IL-5 and IL-13 levels and reduced TGF- β level. Exposure to PM 2.5 alone slightly increased the numbers of dendritic cells (DCs) but reduced the numbers of antigen-presenting cells expressing PD-L1 and Treg cells. Therefore, early exposure to PM 2.5 reduced PD-L1 expression in the lungs of neonatal mice, which interfered with immune tolerance establishment and subsequently resulted in allergic airway inflammation.