N-terminus of Classical swine fever virus strain TD96 glycoprotein E contains a potential heparin-binding domain.

Chih-Yuan Cheng,Ching-Wei Wu,Maw-Sheng Chien,Chienjin Huang

Veterinary Microbiology（2019）

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摘要

•CSFV Erns was codon optimized and expressed in yeast P. pastoris.•CSFV Erns and C-terminally truncated recombinant proteins bound to heparin.•The N-terminal region of CSFV Erns at residues 34－44 contains a potential HBD.•The two basic amino acids K36 and K39 are crucial for binding activity to heparin.

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关键词

Classical swine fever virus,Glycoprotein Erns,Heparin-binding domain,Pichia pastoris,Site-directed mutagenesis

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