CRISPR/Cas9-mediated gene replacement in the basidiomycetous yeast Pseudozyma antarctica.

Emi Kunitake,Takumi Tanaka,Hirokazu Ueda,Akira Endo,Tohru Yarimizu,Etsuko Katoh,Hiroko Kitamoto

Fungal Genetics and Biology（2019）

引用 14|浏览15

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摘要

•We developed an RNP-mediated CRISPR/Cas9 system in the basidiomycete, Pseudozyma antarctica.•Addition of RNP improved both transformation and gene targeting efficiencies.•Highly efficient gene targeting was successful at two loci in the P. antarctica genome.

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关键词

Gene targeting,Homologous recombination,Non-homologous end joining,Ribonucleoprotein,CRISPR/Cas9,Pseudozyma antarctica

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