CRISPR/Cas9-mediated gene replacement in the basidiomycetous yeast Pseudozyma antarctica.
Fungal Genetics and Biology(2019)
摘要
•We developed an RNP-mediated CRISPR/Cas9 system in the basidiomycete, Pseudozyma antarctica.•Addition of RNP improved both transformation and gene targeting efficiencies.•Highly efficient gene targeting was successful at two loci in the P. antarctica genome.
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关键词
Gene targeting,Homologous recombination,Non-homologous end joining,Ribonucleoprotein,CRISPR/Cas9,Pseudozyma antarctica
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