Detection of Ricin in Plant Extracts and Soil Using Liquid Chromatography–High-Resolution Mass Spectrometry
Journal of Analytical Chemistry(2018)
Abstract
A procedure is developed for the detection of ricin in plant extracts and soil based on its hydrolysis with trypsin and chymotrypsin with the formation of specific peptides and their determination by HPLC with high-resolution tandem mass spectrometric detection. The conditions for the sample preparation of plant extracts and soil samples, mass spectrometric detection in the mode of selected reaction monitoring and gradient elution of specific peptides are optimized. The limit of detection of ricin in extracts is 5 μg/mL. The developed procedure is tested on real samples of plant extracts containing natural ricin.
MoreTranslated text
Key words
high-resolution mass spectrometry,high-performance liquid chromatography,ricin,hydrolysis,trypsin,chymotrypsin
AI Read Science
Must-Reading Tree
Example
![](https://originalfileserver.aminer.cn/sys/aminer/pubs/mrt_preview.jpeg)
Generate MRT to find the research sequence of this paper
Chat Paper
Summary is being generated by the instructions you defined