Fc Gamma R-Tlr Cross-Talk Enhances Tnf Production By Human Monocyte-Derived Dcs Via Irf5-Dependent Gene Transcription And Glycolytic Reprogramming

Antigen-presenting cells (APCs) such as dendritic cells (DCs) are crucial for initiation of adequate inflammatory responses, which critically depends on the cooperated engagement of different receptors. In addition to pattern recognition receptors (PRRs), Fc gamma receptors (Fc gamma Rs) have recently been identified to be important in induction of inflammation by DCs. Fc gamma Rs that recognize IgG immune complexes, which are formed upon opsonization of pathogens, induce pro-inflammatory cytokine production through cross-talk with PRRs such as Toll-like receptors (TLRs). While the physiological function of Fc gamma R-TLR cross-talk is to provide protective immunity against invading pathogens, undesired activation of Fc gamma R-TLR cross-talk, e.g., by autoantibodies, also plays a major role in the development of chronic inflammatory disorders such as rheumatoid arthritis (RA). Yet, the molecular mechanisms of Fc gamma R-TLR cross-talk are still largely unknown. Here, we identified that Fc gamma R-TLR cross-talk-induced cytokine production critically depends on activation of the transcription factor interferon regulatory factor 5 (IRF5), which results from induction of two different pathways that converge on IRF5 activation. First, TLR stimulation induced phosphorylation of TBK1/IKK epsilon, which is required for IRF5 phosphorylation and subsequent activation. Second, Fc gamma R stimulation induced nuclear translocation of IRF5, which is essential for gene transcription by IRF5. We identified that IRF5 activation by Fc gamma R-TLR cross-talk amplifies pro-inflammatory cytokine production by increasing cytokine gene transcription, but also by synergistically inducing glycolytic reprogramming, which is another essential process for induction of inflammatory responses by DCs. Combined, here we identified IRF5 as a pivotal component of Fc gamma R-TLR cross-talk in human APCs. These data may provide new potential targets to suppress chronic inflammation in autoantibody-associated diseases that are characterized by undesired or excessive Fc gamma R-TLR cross-talk, such as RA, systemic sclerosis, and systemic lupus erythematous.