Development of a triplex real-time RT-PCR assay for detection and differentiation of three US genotypes of porcine hemagglutinating encephalomyelitis virus.
Journal of Virological Methods（2019）
Abstract
•A triplex real time RT-PCR was developed for detection and differentiation of three genotypes of PHEV.•The developed triplex real time RT-PCR assay showed high specificity and sensitivity.•The triplex real time RT-PCR assay correctly genotyped clinical samples and is useful to monitor PHEV.
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Key words
PHEV,Real time RT-PCR,Genotype,Detection,Differentiation
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