Fragmentation Through Polymerization (FTP): A new method to fragment DNA for next-generation sequencing.
PLOS ONE(2019)
Abstract
Fragmentation of DNA is the very important first step in preparing nucleic acids for next-generation sequencing. Here we report a novel Fragmentation Through Polymerization (FTP) technique, which is a simple, robust, and low-cost enzymatic method of fragmentation. This method generates double-stranded DNA fragments that are suitable for direct use in NGS library construction and allows the elimination of the additional step of reparation of DNA ends.
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Key words
Next Generation Sequencing (NGS),DNA fragmentation,Fragmentation Through Polymerization (FTP),strand-displacement,SD DNA polymerase
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