Dengue-2 virus-like particle (VLP) based vaccine elicits the highest titers of neutralizing antibodies when produced at reduced temperature.

•Dengue structural and non-structural genes are used to form Virus-Like Particles.•Virus-like particles produced at different temperatures display different epitopes.•The distinct dengue virus-like particles are tested as vaccine.•Adjuvanted vaccine produced at 31 °C elicits high titer of neutralizing antibodies.