The effects of herbal extracts on CCl 4 -induced ROS accumulation and cell death in hepatocytes
Oriental Pharmacy and Experimental Medicine(2018)
摘要
The aim of this study was to evaluate the efficacy of herbal medicinal extracts against carbon tetrachloride (CCl 4 )-induced functional alteration and cell death in hepatocytes. HepG2 cells were treated with a frequently utilized herbal medicinal extract, and cell viability and antioxidative activity were subsequently measured with MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide), aspartate transaminase (AST), and alanine transaminase (ALT) assays and MDA (3,4-methylenedioxyamphetamine), superoxide dismutase (SOD), and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assays. Among the extracts from Alnus japonica (ILF-AJ), Angelica keiskei (ILF-AK), Allium tuberosum (ILF-AT), Eucommia ulmoides extract (ILF-E), and Puerariae radix (ILF-P), Rhus verniciflua extract (ILF-R), ILF-E and ILF-R more significantly increased hepatocyte viability in a time- and concentration-dependent manner. Hepatic enzyme activities (AST and ALT), lipid peroxidation, and SOD levels were also significantly regulated in the presence of ILF-E and ILF-R. Next, to assess a synergistic effect between ILF-E and ILF-R, the combined ratio of these two extracts was analyzed. When ILF-E and ILF-R were combined in a 1:1 ratio at a concentration of 100 μg/mL, they more significantly regulated cell death, AST and ALT activity, MDA level, SOD level, and the oxidant capturing characteristic marker DPPH. Through this study, the combined 1:1 formulation of ILF-E and ILF-R is suggested to be a potentially therapeutic and preventive medicinal herb for the preservation of hepatic function and treatment of toxicity.
更多查看译文
关键词
Eucommia ulmoides,Rhus verniciflua
AI 理解论文
溯源树
样例
![](https://originalfileserver.aminer.cn/sys/aminer/pubs/mrt_preview.jpeg)
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要