Development and validation of markers for spike density QTL, Qsd.sau-7A from Tibetan semi-wild wheat (Triticum aestivum ssp. tibetanum)

Spike density is one of the few traits that determines the yield potential in wheat. Three high resolution melting (HRM) markers (0C32-715, DCE-464, and C81A-240) were developed and used to map the QTL for spike density Qsd-sau-7A. The markers 0C32-715 and DCE-464 were validated in two of the three validation population. DCE-464 and 0C32-715 were the most closely linked markers that could be used to introgress QSd.sau-7A through molecular maker assisted breeding and development of near isogenic lines.