Characterization of strawberry (Fragaria vesca) sequence genome

bioRxiv(2018)

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摘要
Background: In order to understand strawberry genes structure and evolution in this era of genomics, it is important to know the general statistical characteristics of the gene, intron and exon structures of strawberry and the expression of genes on different parts of strawberry genome. In the present study, about 32,422 genes on strawberry chromosomes were evaluated, and a number of bioinformatic softwares were used to analyze the characteristics of genes, exons and introns, expression of genes in different regions on the chromosomes. Also, the positions of strawberry centromeres were predicted.Results: Our results showed that, there are differences in the various features of different chromosomes and also vary in different parts of the same chromosome. The longer the number of genes, the longer the length of chromosome. The average length of genes is about 2809bp and the length of the individual gene is 0-2000bp with 5.3 exons and 4.3 introns per gene. The average length of the exon was 229bp and the intron was 413bp. Among the evaluated genes, ehe intronless gene accounted for 20.05%. Consistently a same trend with the expression levels of the same parts of the gene on a chromosome in different organizations was observed. Finally, the number of genes was positively correlated with the number of intronless, and there was a negative correlation of the length of the gene. The length of the gene depends primarily on the length of the intron, and the length of the exon has little effect on it. The number of exons was negatively correlated with the length of the exons, and the intron was also true.Conclusion: The results of this investigation could definitely provide a significant foundation for further research on function analysis of gene family in Strawberry.
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关键词
strawberry,exon,intron,Structural features,Correlation analysis,gene expression
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