MicroRNA-125b-1-3p mediates intervertebral disc degeneration in rats by targeting teashirt zinc finger homeobox 3.

The present study aimed to investigate the association between Teashirt zinc finger homeobox 3 (TSHZ3) and the nucleus pulposus (NP) of intervertebral discs in rats. TSHZ3 was identified from the differentially expressed micro (mi) RNAs in the expression profile of GSE63492 by identifying the overlapped target genes of microRNA (miR)-125b-1-3p across different databases. TSHZ3 small interfering RNA (siRNA) and an miR-125b-1-3p inhibitor were used for gene silencing and gene silencing efficiency was assessed by reverse transcription-polymerase chain reaction. Western blotting was performed to detect the cell cycle proteins cyclin D1 and B1 and the proteins associated with DNA damage in NP. The results revealed that in normal NPs, the expression of TSHZ3 increased following the inhibition of miR-125b-1-3p and in DNA damaged NPs, the expression of TSHZ3 was associated with the degree of DNA damage. The present study demonstrated that TSHZ3, as a target gene of miR-125b-1-3p, may serve a protective role in intervertebral disc degeneration and that this protective function may be inhibited by high levels of miR-125b-1-3p.