In vitro reconstitution of translational arrest pathways.
Methods（2018）
Abstract
•Cell-free systems enable mechanistic analyses of translational arrest pathways.•Rabbit reticulocyte lysate is an efficient mammalian in vitro translation system.•Biochemical strategies can isolate specific ribosome-nascent protein complexes.•Arrest pathways can be reconstituted using top-down and bottom-up approaches.
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Key words
RRL,IVT,RNC,rpm
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