MFHAS1 suppresses TLR4 signaling pathway via induction of PP2A C subunit cytoplasm translocation and inhibition of c-Jun dephosphorylation at Thr239

•The expression of MFHAS1 is significantly increased after activating TLR4 signaling pathway.•MFHAS1 inhibits expression of inflammatory factors and plays a role in negatively regulating TLR4 signaling pathway.•MFHAS1 induces cytoplasm translocation of C subunit of PP2A and decreases dephosphorylation of c-Jun at Thr239.