Assessment of Genetic Diversity Using ISSR Markers in Green Gram [Vigna radiata (L.) Wilczek]

Molecular characterization is helpful in understanding the phylogenetic relationship among various germplasm to reveal the genetic diversity among the used parental genotypes.Among several efficient methods for revealing genetic variability within and among plant populations, one of the most widely applied methods is ISSR marker analysis.ISSR, markers are commonly used because they are quick, simple and environment non-sensitive enabling genetic diversity analysis in several types of plant material like natural populations, population in breeding programmes.Evaluation of genetic diversity would promote the efficient use of genetic variations, effective conservation and purity of the genotype to be determined as well as utilization of germplasm in crop improvement.ISSR marker analysis was performed to detect relatedness and diversity among eight parental genotypes.ISSR markers are useful in detecting polymorphism among accessions by generating a large number of markers that target multiple microsatellite loci distributed across the genome.Out of 109 scorable bands, 88 bands were polymorphic and the level of polymorphism was 81 per cent.Twenty five ISSR primers were used, out of which eighteen primers showed amplification in all genotypes.The average number of bands per primer was found to be 6.22 and average numbers of polymorphic bands per primer were 4.89.ISSR-01 proved to be best primer in the present investigation with total 29 fragments and eight highest scorable bands as well as 100 per cent polymorphism. K e y w o r d sMungbean, ISSR Markers, Yield and yield components.