Genome‑wide profiling of lncRNA and mRNA expression in CRSwNP.

Chronic rhinosinusitis with nasal polyps (CRSwNP) is one of the most prevalent chronic diseases. In patients with CRSwNP, the present study performed comprehensive bioinformatics analyses to characterize the transcriptome profiles of mRNAs and long non-coding RNAs (lncRNAs). A total of 265 differentially expressed lncRNAs and 994 mRNAs were identified. The majority of up- and downregulated differentially expressed genes were significantly enriched in the biological process of signal transduction'. The most significantly enriched molecular function was protein binding' and the most significantly enriched cellular component was membrane'. Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis led to identification of several significantly enriched pathways [false discovery rate (FDR)<0.05], including cytokine-cytokine receptor interaction' (FDR=3.94x10(16)) and cell adhesion molecules' (CAMs) (FDR=1.28x10(-5)). Key differentially expressed lncRNAs were identified, including lncRNA XLOC_010280, which regulates chemokine (C-C motif) ligand 18 (CCL18) and inflammation, and RP11-798M19.6, which regulates polypeptide N-acetylgalactosaminyltransferase 7 (GALNT7) and cell proliferation. Based on the results of reverse transcription-quantitative polymerase chain reaction, except for CCL8, neural precursor cell expressed developmentally downregulated gene 4-like and GALNT7, the expression of 3 other selected genes was consistent with the results of integrated analysis. The results of the present study provide a foundation for future investigations into mRNAs and lncRNAs as diagnostic and therapeutic targets in CRSwNP.