Paraliobacillus Zengyii Sp. Nov., A Slightly Halophilic And Extremely Halotolerant Bacterium Isolated From Tibetan Antelope Faeces

Two rod-shaped, slightly halophilic and extremely halotolerant bacterial strains (X-1125(T) and X-1174), which were Gramstain-positive, facultatively anaerobic and motile with peritrichous flagella, were isolated from the faeces of Tibetan antelopes. Their optimal temperature, NaCl concentration and pH for growth were 28 degrees C, 3% (w/v) NaCl and pH 7.5, respectively. Based on the results of 16S rRNA gene sequences, and phylogenetic and phylogenomic analyses, their nearest phylogenetic neighbours were Paraliobacillussediminis KCTC 33762(T) (98.4 % similarity), Paraliobacillusguinghaiensis CGMCC 1.6333(T) (96.9 %) and Paraliobacillusryukyuensis NBRC 100001(T) (95.9 %) while the 16S rRNA genes of strains X-1125(T) and X-1174 were highly similar (99.7 %) to each other. The polar lipids comprised diphosphatidylglycerol, two unidentified phospholipids and four unidentified lipids. MK-7 was the sole menaquinone (100 %). The cell wall contained alanine, glycine, glutamic acid and meso-diaminopimelic acid. The major fatty acids (>9%) were anteiso-C-15:0, anteiso-C-17(:0) and C-16:1 omega 11c. The in silico DNA-DNA hybridization value between strains X-1125(T) and X-1174 was 97.8 % (well above the species threshold), but their values were lower than the 70 % threshold with the three closely related type strains. Strains X-1125(T) and X-1174 had DNA G+C contents (mol%) of 35.2 and 35.1 %, respectively. Based on the presented data, strains X-1125(T) and X-1174 hereby represent a novel species of the genus Paraliobacillus, for which the name Paraliobacillus zengyii sp. nov. is proposed. The type strain is X-1125(T) (=DSM 107811(T)=CGMCC 1.16464(T)).