Development of Leishmania donovani stably expressing DsRed for flow cytometry-based drug screening using chalcone thiazolyl-hydrazone as a new antileishmanial target
International Journal of Antimicrobial Agents(2016)
Abstract
•Leishmania donovani expressing DsRed were generated using vector template DsRed-Express-N1.•Stable transfectants showed enhanced expression of DsRed and high infectivity to J774.1 macrophages.•Transfectants were used in flow cytometry-based antileishmanial drug screening.•Drug screening using standard antileishmanials and a new chemical series of chalcone thiazolyl-hydrazone hybrids.
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Key words
Red fluorescent protein,DsRed-Express-N1,Multiparameter flow cytometry,Leishmania donovani,Mean fluorescence intensity,Chalcone thiazolyl-hydrazone
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