GW26-e4401 MiR-195-3p/-5p decrease cardiac fibroblast proliferation and the transdifferentiation into myofibroblasts

RESULTS Microarray results showed that after 48 hours, 4343 gene transcripts differed (p<0.05) between controp group and HCY group. Fuctional analysis showed a significant number of genes to be associated with apoptosis, cell cycle pathway, enzyme, immunity protein, microtubular dynamic, nucleic acid binding,signal transducer. 3409 gene transcripts were up-regulated while 121 gene transcripts were down-regulated in HUVEC treated with TXL. These gene transcripts have been proved to relate to biological process, molecular function and cellular structure. Significant changes were observed in genes involved in apoptosis, cellular proliferation, cell signal transduction and oxidative stress. including vescular endothelial growth factor (VEGF), IGF receptors, p42/44 mitogen activated protein (MAP) kinase, endothelial nitric oxide synthase (eNOS), chemokine (C-C motif) ligand 2 (CCL2), chemokine (C-C motif) ligand 5 (CCL5), chemokine (C-X-C motif) ligand 10 (CXCL10), and toll like receptor 3 (TLR3).