P3‐044: Mild Beta‐Amyloid Preconditioning has a Neuroprotective Effect by Enhancing Cellular Tolerance VIA BDNF Pathway

Since Janoff introduced novel strategy in 1964, preconditioning has been an attractive strategy for protecting neurons by enhancing cellular tolerance. Abundant studies have shown possible links between low levels of brain-derived neurotrophic factor (BDNF) and neurodegenerative diseases such as Parkinson’s disease, Huntington’s disease, and Alzheimer’s disease (AD). Recombinant BDNF has protective functions on in vitro and in vivo AD models. We cultured primary rat neuron cell with 100nM Aβ for preconditioning. MTT(2.5mg/ml) assay was performed to measure cell viability. Western blotting; We detected cell survivor pathway protein and measured mitochondria potential by using JC-1. 100nM Aβ42 Preconditioning enhanced cellular tolerance and increased cell viability than control group at 1μM Aβ toxicity. Aβ42 Preconditioning maintained mitochondria potential and prevented cell apoptosis. This study indicates that mild Aβ Preconditioning activates cell survivor mechanism and prevents apoptosis result from Aβ toxicity. This survivor maybe arise from BDNF pathway.