Thin-layer chromatographic method optimization of a Piper guineense extract: comparison with published methods

Piper guineense Schumach is a medicinal plant that contains piperine as its major bioactive compound. Piperine alkaloid has antidepressant like properties [1]. The analysis of piperine is usually performed by thin layer chromatography (TLC), a method widely used in the analysis of bioactive compounds for drug discovery. However, the TLC method used for the separation of P. guineense extracts lacks optimization of the mobile phase and the effects of the developing chamber on the separation. The aim of the study was to develop an optimal TLC method for the separation of P. guineense extracts.