旋毛虫排泄分泌蛋白对人肝癌HepG2细胞增殖的抑制作用
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi（2015）
Abstract
采用MTT法检测旋毛虫肌幼虫排泄分泌蛋白对人肝癌HepG2细胞增殖的抑制作用.AO/EB荧光染色观察与300 μg/ml排泄分泌蛋白共培养24 h的HepG2细胞的细胞核形态.用75 μg/ml排泄分泌蛋白作用HepG2细胞24 h后,流式细胞术(FCM)分析细胞凋亡率,免疫荧光法检测细胞cleaved-caspase 9的表达.结果显示,旋毛虫排泄分泌蛋白呈剂量依赖性抑制人肝癌HepG2细胞的增殖.300 μg/ml排泄分泌蛋白作用24 h后,HepG2细胞核固缩呈圆珠状凋亡特征.75 μg/ml排泄分泌蛋白作用细胞24 h后,细胞早期和晚期凋亡率分别为17.9％和7.3％,细胞坏死占6.6％;HepG2细胞中cleaved-caspase 9荧光强度明显高于阴性对照.
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Key words
Trichinella spiralis,Excretory/secretory protein,Hepatocellular carcinoma HepG2 cell line,Apoptosis
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