OP0168 Nik-ikk complex interaction controls nf-Κb-dependent inflammatory activation of the endothelium in response to ltΒr ligation
JOURNAL OF CELL SCIENCE(2018)
摘要
NE-kappa B-inducing kinase (NIK; also known as MAP3K14) is a central regulator of non-canonical NF-kappa B signaling in response to stimulation of TNF receptor superfamily members, such as the lymphotoxin-beta receptor (LT beta R), and is implicated in pathological angiogenesis associated with chronic inflammation and cancer. Here, we identify a previously unrecognized role of the LT beta R-NIK axis during inflammatory activation of human endothelial cells (ECs). Engagement of LT beta R-triggered canonical and non-canonical NF-kappa B signaling promoted expression of inflammatory mediators and adhesion molecules, and increased immune cell adhesion to ECs. Sustained LT beta R-induced inflammatory activation of ECs was NIK dependent, but independent of p100, indicating that the non-canonical arm of NF-kappa B is not involved. Instead, prolonged activation of canonical NF-kappa B signaling, through the interaction of NIK with I kappa B kinase alpha and beta (also known as CHUK and IKBKB, respectively), was required for the inflammatory response. Endothelial inflammatory activation induced by synovial fluid from rheumatoid arthritis patients was significantly reduced by NIK knockdown, suggesting that NIK-mediated alternative activation of canonical NE-kappa B signaling is a key driver of pathological inflammatory activation of ECs. Targeting NIK could thus provide a novel approach for treating chronic inflammatory diseases.
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关键词
NE-kappa B-inducing kinase,NIK,Lymphotoxin-beta receptor,LT beta R,Inflammation,Endothelial cells
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