Discovery Of Stealthin Derivatives And Implication Of The Amidotransferase Flsn3 In The Biosynthesis Of Nitrogen-Containing Fluostatins
MARINE DRUGS(2019)
摘要
Diazobenzofluorene-containing atypical angucyclines exhibit promising biological activities. Here we report the inactivation of an amidotransferase-encoding gene flsN3 in Micromonospora rosaria SCSIO N160, a producer of fluostatins. Bioinformatics analysis indicated that FlsN3 was involved in the diazo formation. Chemical investigation of the flsN3-inactivation mutant resulted in the isolation of a variety of angucycline aromatic polyketides, including four racemic aminobenzo[b]fluorenes stealthins D-G (9-12) harboring a stealthin C-like core skeleton with an acetone or butanone-like side chain. Their structures were elucidated on the basis of nuclear magnetic resonance (NMR) spectroscopic data and X-ray diffraction analysis. A plausible mechanism for the formation of stealthins D-G (9-12) was proposed. These results suggested a functional role of FlsN3 in the formation/modification of N-N bond-containing fluostatins.
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关键词
angucyclines,N-N bond,raceme,gene inactivation,biosynthesis,marine,Micromonospora
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