Effect Of Combination Therapy Of Propofol And Sevoflurane On Map2k3 Level And Myocardial Apoptosis Induced By Ischemia-Reperfusion In Rats
INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE(2015)
摘要
To investigate the mechanism of combination therapy of propofol and sevoflurane on MAP2K3 level and myocardial apoptosis induced by ischemia-reperfusion (IR) in rat. A total of 30 SD rats were randomly separated into 3 groups: normal, IR (ligation of left coronary artery), and IR+ propofol and sevoflurane (IR+P+S). Different methods were used to detect the serum index associated IR injury. TUNEL assay was used to analyze the apoptotic cells of rat heart tissues. qRT-PCR was used to analyze the mRNA levels of cell apoptosis related proteins such as Bcl-2, Bax, and MAP2K3. Western blotting was used to detect the expression of Bcl-2, Bax, MAP2K3, and Caspase-3 of heart tissues. Compared with normal group, serum LDH, cTnI, and CK-MB levels in IR group were significantly increased with time increasing (P < 0.05), while that in IR+P+S group were significantly decreased compared with that in IR group (P < 0.05). The percentage of apoptotic cells of heart tissue in IR+P+S group was larger than that in IR group (P < 0.05). Compared with IR group, mRNA expression of MAP2K3 and Bax were significantly decreased with Bcl-2 was significantly increased in IR+P+S group (P < 0.05). Also, expression of MAP2K3, Caspase-3, and Bcl-2 in IR+P+S group were statistically lower while Bax was statistically higher than that in IR group (P < 0.05). Our study suggested that combination therapy of propofol and sevoflurane may protect myocardial cells from damage during IR through decreasing MAP2K3 level and reducing cell apoptosis via Bcl-2/Bax pathway.
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关键词
Ischemia-reperfusion, MAP2K3, cell apoptosis, propofol and sevoflurane
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