光色素荧光探针测定抗体蛋白质
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)(2010)
Abstract
由于牛血清白蛋白(BSA)与光色素的结合反应,引起光色素荧光淬灭,且其荧光淬灭程度与BSA的浓度在0.050~1.0 mg.L-1范围内呈线性关系。据此提出了以光色素为探针测定抗体蛋白质的荧光方法。方法的检出限(3S/N)为8.0μg.L-1。用标准加入法对方法的回收率作了试验,测得回收率在95.0%~104.0%之间,测定值的相对标准偏差(n=6)小于2.5%。
MoreTranslated text
Key words
Fluorescence probe,BSA,Antibody protein,Fluorospectrophotometry,Lumichrome
AI Read Science
Must-Reading Tree
Example
![](https://originalfileserver.aminer.cn/sys/aminer/pubs/mrt_preview.jpeg)
Generate MRT to find the research sequence of this paper
Chat Paper
Summary is being generated by the instructions you defined