Comparative Study on Three Kinds of DNA Extraction Methods of Salmonella and Staphylococcus aureus

[Objective] To evaluate different extraction methods used for Salmonella and Staphylococcus aureus genome DNA.[Method] Three kinds of methods have been applied to extract the DNA from Salmonella and Staphylococcus aureus. The concentration and purity were compared using full wavelength ultraviolet / visible light scanning spectrophotometer.[Result]The quality of DNA extrated by using method 3 was superior to the other methods,also,he concentration and purity of DNA was relatively high. As to the DNA extracted by method 1,its purity was no less than which extracted by method 3 while concentration is lower. So the quality of DNA extracted by using method 1 was estimated to be second only to method 3. Extrating DNA by using method 2 seemed fine,but the fouling of carbohydrates or salts has come into being a question. [Conclusion] Method 3 of this study is effective and generally applicable to Salmonella,Staphylococcus aureus and other pathogenic bacteria for DNA extraction.