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CMC-Na法提取牦牛血红素的工艺研究

LIU Zhen-bin,ZHANG Zhen,ZHANG Sheng-gui,NIU Li-li,ZHOU Yun,HU Wei,PANG Chen

Journal of Gansu Agricultural University（2012）

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Abstract
以新鲜牦牛血为原料,采用超声波破碎进行溶血,利用羧甲基纤维素(CMC)的吸附作用提取血红素.结果表明:提取牦牛血红素的最佳的条件为:10mL红血球中,去离子水添加量为40mL,超声波破碎时间为20min,分离pH值为2.0,CMC-Na(3%)的添加量为3mL,该工艺从牦牛血全血中提取的血红素产率为3.028mg/mL.
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Key words
hemoglobin,CMC-Na,yak
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