Primary study of the role of alloreactive natural killer cell on the transfusion-associated immunodepression

Objective To investigate the role of alloreactive natural killer cell on the immunological suppression of blood transfusion when blood donors KIR and receptor's HLA-Ⅰ molecular in varying degree matching situations.Methods NK cells from PBMC were isolated by using miniMACS and NK Cell Isolation KitⅡ in the simulated clinical external blood transfusion,then cultured in vitro.Five kinds of inhibitory KIRs were genotyped by PCR-SSP method.NK cells(4 ml a total of 1×106 cells) known KIR types were mixed with the blood(20 ml) known HLA-I type in certain proportion(equal to inputs the 800ml whole blood to person who is 60 kilogram body weight),samples were taken on different times point and the immunologic changes were detected.A total of 20 groups were analyzed on the degree of mismatching of KIR and HLA.The degree of mismatching on the immune function of blood was compared.Results Based on the analysis of the immunologic indexes of different level of KIR and HLA,mixed and incubated from 0h to 24h,we found that the concentration of IL-2、 IL-4、 PGE2 、TNF-α、TNF-β、IFN-γ and GM-CSF were increased form(1.97±0.12)、(0.22±0.03)、(0.22±0.06)、(0.19±0.02)、(0.13±0.02)、(0.12±0.02)、(0.19±0.02)μg/L to(2.94±0.06)、(0.55±0.09)、(0.53±0.05)、(3.54±0.07)、(3.08±0.14)、(2.65±0.11)、(2.37±0.16)μg/L(P0.05);the concentration of IgA、IgM and IgG were decreased from(1.20±0.20)、(1.28±0.07)、(9.89±0.37)g/L to(0.60±0.07)、(0.72±0.06)、(6.66±0.91)g/L(P0.05) at different levels.There is no correlation between KIR-HLA matching level with the concentration changing of IL-2、IL-4、PGE2、IgA、IgM、IgG(P 0.05).However,the concentration of TNF-α、TNF-β、IFN-γ and GM-CSF were increased from(0.42±0.04)、(0.37±0.04)、(0.46±0.08)、(0.43±0.02)μg/L to(3.54±0.07)、(3.08±0.14)、(2.65±0.11)、(2.37±0.16)μg/L(P0.05) with the raising of mismatching degree between KIR and HLA(P0.05).Conclusion The higher degree of mismatching of KIR and HLA is,the more can stimulate the immunological function of alloreactivity of NK cells.It will be better to reverse the transfusion-associated immunodepression.