油菜叶片中硫甙总量与分量的HPLC测定
Journal of Instrumental Analysis(2006)
Abstract
研究建立了高效液相色谱测定油菜叶片中硫甙总量与分量的测定方法。油菜叶片经20 h冰冻干燥,70%(φ)甲醇为提取溶剂,超声波提取18 m in,1 mg硫酸酯酶脱硫酸根,离子交换树脂纯化,高效液相色谱进行油菜叶片硫甙组分定性、定量。该方法加标回收率为94%~103%,样品极差为2.29μmol/g,符合ISO9167国际标准要求。
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Key words
HPLC,Glucosinolate,Rapeseed leaves
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