A new real-time RT-qPCR assay for the detection, subtyping and quantification of human respiratory syncytial viruses positive- and negative-sense RNAs
Journal of Virological Methods(2016)
摘要
•New quantitative RT-qPCR assays for RSV-A and -B were developed.•Both assays allow rapid detection, quantification and subtyping of RSV.•The efficiency test was validated with in vitro transcripts and clinical samples.•Comparable sensitivity was observed with RSV positive and negative sense RNAs.•The assays provide a useful tool for both RSV diagnostics and research.
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关键词
6-FAM,BHQ,CT,hMPV,hPIV,L-LOD,LNA,MGB-NFQ,QCMD,RSV,RT-qPCR,U- and L- LOQ
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