Influence of GM_1 on the Cerebral Cortex and Hippocampus GAP-43 Expression in the Cerebral Trauma Rats

Objective To observe the effects of the ganglioside(GM1) on brain trauma cerebral cortex and hippocampus nerve growth associated protein(GAP-43) expression in rats, to explore the possible mechanisms of GM1 promoting the nerve repair of traumatic brain injury. Methods200 male SD rats(10 weeks old), were used in this experiment. Of which 60 mice were randomly divided into sham group, no brain trauma, preoperative intraperitoneal injection of saline3 d. 140 mice were used as traumatic brain injury model, divided into model group 70 mice,preoperative intraperitoneal injection of saline 3 d; GM1 group 70 mice, preoperative intraperitoneal injection GM13 d. ELISA was used to detect parietal cerebral cortex and hippocampus of GAP-43 expression at 0~24 h at different time, and after 1~5 d at different time take the behavioral testing. Results(1) 0~16 h GAP-43 expression in rat brain GM1 parietal cortex and 0~24 h hippocampus were significantly lower than the model group(P0.01), and compared with the sham group, there was no significant difference(P0.01).(2) GM1 rat parietal cortex GAP-43 expression, postoperative increase over time and reached the level of the untreated group at 24 h.(3) Conducts in sham group 1~5 d school performance was better than these in model group and GM1 group(P0.01), GM1 group gradual increase in exercise capacity after 2~5 d, behavioral performance was better than that in the model group(P0.05). Conclusions Prophylactic use of GM1 can effectively promote brain cortex GAP-43 expression and reduce the extent of brain damage in rats with traumatic brain injury, which plays its neuroprotection roles in experimental traumatic brain injury in rats.