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壳寡糖含量的测定方法比较

Chinese Journal of Pharmaceutical Analysis(2011)

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Abstract
目的:寻找一种简便有效测定壳寡糖的方法.方法:采用Elson - Morgan法、苯酚-硫酸法和3,5-二硝基水杨酸(DNS)法测定壳寡糖含量并进行比较.结果:Elson - Morgan法不适用于壳寡糖含量的测定;苯酚-硫酸法中盐酸氨基葡萄糖在200~3000 μg范围与吸光度也有很好的线性关系,但苯酚-硫酸法对壳寡糖的测定值较实际值偏大;DNS法测定壳寡糖与实际值接近,平均回收率为96.1%,RSD为2.9%(n=6).结论:DNS法操作简单、快速,重现性好,可直接用于壳寡糖含量的测定.
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