外源性FHIT基因转染对人胃癌细胞系MKN-45迁移和侵袭能力的影响
Journal of Southeast University(Medical Science Edition)(2009)
Abstract
目的:探讨脆性组氨酸三联体(FHIT)基因转染对人胃癌细胞MKN-45迁移和侵袭能力的影响。方法:将载有人外源性FHIT基因的真核表达质粒pcDNA3.1-FHIT,转染至FHITmRNA阴性表达的人胃癌细胞MKN-45,分别应用Millicell小室细胞迁移实验、Transwell小室侵袭实验检测转染前后MKN-45细胞迁移能力和侵袭力的改变。结果:外源性FHIT基因转染MKN-45细胞后,MKN-45细胞的迁移能力明显降低(P<0.05),但细胞侵袭能力无明显变化(P>0.05)。结论:外源性FHIT基因转染致MKN-45细胞系的迁移能力明显降低,对MKN-45细胞系的侵袭能力无明显影响。
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Key words
migration,gastric cancer,transfection,fragile histidine triad gene,invasion
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