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HPLC-ELSD法测定巴戟天有效成分巴戟甲素含量

LIU Xiaohan,XIAO Fengxia,CHEN Yonggang,LIN Li,LIN Shaodong,QIU Ronghua,LIU Zheng

Traditional Chinese Drug Research & Clinical Pharmacology（2009）

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Abstract
目的建立HPLC-ELSD法测定巴戟天中巴戟甲素的含量。方法色谱柱:Columns InertsilNH2(4.6m m×250m m,5μm);柱温:室温;流动相:甲醇-水(20∶80);流速:1.0mL.min-1;漂移管温度:104℃;N2流速:2.9L.min-1。结果巴戟甲素进样量在2μg～20μg范围内有良好的线性关系,r=0.9996,平均回收率为98.1%(n=9),RSD为1.8%。结论该法灵敏、准确,重复性好,可用于巴戟天中巴戟甲素含量测定及其质量控制。
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Key words
Bajijiasu,Morinda officinalis How,Content determination,HPLC-ELSD
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