Screening of endophytic fungi from Curcuma wenyujin with anti-inflammatory and anti-oxidant activities

Objective To study the anti-inflammatory and anti-oxidant activities of endophytic fungi from medicinal plant Curcuma wenyujin,to obtain the strains with good anti-inflammatory and anti-oxidant activities,and to find the new substances with anti-inflammatory and anti-oxidant activities.Methods The anti-inflammatory activities of 50 strains from the isolated endophytic fungi were tested by animal models with ear swelling induced by xylene and peritoneal capillary permeability induced by acetic acid in mice;DPPH method was used to evaluate the anti-oxidant activities of endophytic fungi.Results Twenty-one strains of endophytic fungi could inhibit ear swell induced by xylene in mice and reduce peritoneal capillary permeability of mice induced by acetic acid.Compared with the group of physiological saline,the groups of E3,E8,E17,E24,E37,and E4 had the significant inhibitory effects(P 0.01),which was the same as Naproxen;Eight strains of endophytic fungi had the anti-oxidant activity.There was a dose-effect relationship between the fermentation products and the anti-oxidant activity,and their free radical scavenging rates against DPPH were above 90% when the concentration of E2,E17,and E37 were at 140 μg/mL.Conclusion Some endophytic fungi from C.wenyujin have the anti-inflammatory and anti-oxidant activities,strains E2,E3,E8,E17,E24,E37,and E46 show the significant effect on the anti-inflammatory and anti-oxidant activities,which has a perspective in research and development.